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. 2020 Sep 9;51:112. doi: 10.1186/s13567-020-00836-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Serum neutralization (SN) titers and the level of IFN-γ in mice immunized with various immunogens. A The SN titers in mice induced by 4Cap(d41) and commercial vaccines. 5 × 10⁷ Sf-9 cells were infected with the recombinant baculovirus BacDD-4Cap(d41) at an MOI of 10 for 3 days. Cell lysates were collected to prepare the 4Cap(d41) vaccine. Eight-week-old mice were vaccinated via intraperitoneal injection at weeks 0 and 2 with PBS, CE, 4Cap(d41), and commercial vaccines as formulated with complete and incomplete adjuvants. As negative controls, three mice were injected with PBS and CE (5 × 10⁷ BacCE-infected Sf-9 cell lysates). Each mouse received one booster shot in week 2, and blood samples were taken at week 5 for the SN titer assay. B The fluorescence results were converted into neutralizing antibody titers and presented as a data chart (B). Data represent the mean ± SD. C Analysis of IFN-γ by ELISA was performed using the IFN-γ ELISA Kit. The value of OD₄₀₅ nm was measured with an ELISA reader. Data represent the mean ± SD. A P value less than 0.05 was considered significant.