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. 2020 Sep 9;51:112. doi: 10.1186/s13567-020-00836-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Quantification of PCV2 viral loads in sera from the challenged pigs. 2-month-old SPF pigs were immunized at the base of the ear by the intramuscular route with CE, 4Cap(d41), and commercial vaccines, respectively. Serum samples were collected every week after the first immunization. In the eighth week after the first immunization, all vaccinated pigs were challenged via intranasal injection with 2 ml of PCV2 (10^5.7TCID50/mL). The 8^th week after the first immunization is designated as the 0^th week. PCV2 viral loads were detected by real-time quantitative PCR one week post challenge. Data are presented as mean ± SD. Viral loads were determined as the mean of the logarithmic DNA copy number per ml (log copies/ml). An asterisk (*) shows a statistically significant difference between the indicated groups (P < 0.05).