Table 1.
Primer pairs used in this study
| Primer name | Sequence in 5′–3′ direction | Location (nt) | Accession number | Product name | Length (bp) |
|---|---|---|---|---|---|
| Cap(d41)-XhoI (Forward) |
XhoI AGCCTCGAGATGACGTATCCAAGG |
124–702 | AY885225 | Cap(d41) | 579 |
| Cap(d41)-BsiWI (Reverse) |
PstI TCATCTGCAGTGAGGGTTTAA GTG |
||||
| Cap-(d41)-NotI (Forward) |
NotI GCGGCCGCTATGAATGGCATC TTCAACACC |
124–702 | AY885225 | Cap(d41) | 579 |
| Cap(d41)-SalI (Reverse) |
SalI GTCGACTATGGGTTTAAGTGG GGGGTC |
||||
| Cap(d41)-XhoI (Forward) |
XhoI CTCGAGATGAATGGCATCTTC AACACC |
124–702 | AY885225 | Cap(d41) | 579 |
| Cap(d41)-BsiWI (Reverse) |
BsiWI CGTACGTATGGGTTTAAGT GGGGGGTC |