Fig. 2.

Visualization of KA-induced Bdnf expression. a. Representative images of in vivo BLI 6 h after administration of saline or KA. b. ROI analysis. Data represent the mean ± SEM of three independent experiments using the unpaired t test (*p < 0.05). c. RT-PCR analysis. After in vivo BLI, total RNA was prepared from the hippocampus (Hp) and cerebral cortex (Cx) of Bdnf-Luc mice to examine changes in endogenous Bdnf mRNA levels. Data represent the mean ± SEM of three independent experiments using the unpaired t test (*p < 0.05, NS; not significant). d. Immunoblot analysis. After in vivo BLI, proteins were extracted from the hippocampus and cerebral cortex of Bdnf-Luc mice to examine changes in endogenous BDNF protein levels. e. The intensities of bands shown in Fig. 2d were quantified using Image J. Data represent the mean ± SEM of three independent experiments using the unpaired t test (**p < 0.01, NS; not significant)