Fig. 4.

Visualization of sensory stimulation-induced Bdnf expression. a. Schedule of experiments (also refer to the Materials and methods). b. Representative images of in vivo BLI. In vivo BLI was performed 6.5 d after houseing Bdnf-Luc mice in the dark [Light (−)]. The mice were housed in the dark for an additional 2 d, and were then exposed to light for 1 h. After light exposure for 1 h, the mice were again housed in the dark for 5 h, and then in vivo BLI was performed [Light (+)]. c. ROI analysis. ROI V1 and V2 contains the visual cortex, and ROI S1 and S2 contains the somatosensory cortex (also refer to Supplementary Fig. 3 in detail). Data represent the mean ± SEM of four independent experiments using the paired t test (*p < 0.05, NS; not significant)