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. 2020 Sep 7;19:178. doi: 10.1186/s12934-020-01432-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Detection of CDPs and their derivatives in culture supernatants of E. coli bacteria overexpressing CDPS and CDO. Culture supernatants of E. coli bacteria overexpressing no enzyme (a) or a CDPS/CDO couple from one pathway (b, N. potens; c, pathway 1 of A. oligospora; d, S. catenulae; e, S. rimosus; f, S. sp. NRRL 5123; g, pathway 2 of A. oligospora; h, S. aidingensis) were collected, treated by SPE, and analysed by LC–MSMS. UV traces recorded at 214 nm are shown between 16 and 31 min. The Y axes of the UV₂₁₄ traces were set from 20 to 280 mAU. Recovered CDPs and the m/z values of their MH⁺ ions are indicated in grey. Stars indicate CDPs that have not been previously described for the characterized CDPSs. The m/z values of detected MH⁺ parent ions corresponding to dehydrogenated CDPs (loss of 2 and 4 atomic mass units) are shown in brown. The experiment was performed three times with similar results. Compounds chosen for purification and NMR characterization are indicated in black bold Arabic numbers in brackets (1–5)