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. 2020 Sep 5;39:179. doi: 10.1186/s13046-020-01687-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — PMSB8-AS1 promotes PC cell proliferation and metastasis in vitro. a The qRT-PCR was performed to confirm the transfection efficiency of PSMB8-AS1 overexpressed and underexpressed lentivirus plasmid in PANC-1 and MIA-paca2 cells. b CCK-8 assay of PSMB8-AS1 knockdown and control group PC cells at the indicated times. c Plate clone formation assays were performed to evaluate cell invasion in PSMB8-AS1 overexpressed and knockdown PC cells. d Cell scratch assay for the migration ability of the indicated PC cells. e Transwell experiments were performed to analyze the cell migration and invasion in PSMB8-AS1 overexpressed and knockdown PC cells. f Western blot analysis to determine expression level of the EMT and cell cycle relative marker in PSMB8-AS1 overexpressed and knockdown PC cells. g Effects of PSMB8-AS1 overexpression and knockdown on cell cycle progression in PC cells