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. 2020 Sep 7;8:158. doi: 10.1186/s40478-020-01028-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Pan-neuronal expression of 1000 repeat DPRs alters longevity but not viability. a Mating scheme for Drosophila viability assays. Flies homozygous for the UAS-GFP-DPR constructs were crossed to flies carrying the pan-neuronal driver (nSyb-Gal4) over the Tm6b balancer chromosome. Normal mendelian inheritance should result in a 50:50 ratio of flies carrying both the driver and DPR (pan-neuronally driven DPR) and those carrying only the DPR in the absence of the driver (undriven siblings). b pan-neuronal expression of each DPR line following the mating scheme in “a” shows no significant variance in viability compared to GFP (mCD8-GFP) controls or to the expected 50:50 ratio. Two independent lines of each DPR are shown. N is shown above each bar. c flies pan-neuronally expressing GR1000 show a significant reduction in longevity compared to wild type and control flies (Survival Log-Rank (Mantel-Cox) with Bonferroni Correction, *** p < .001 (See Additional file 1: Online resource 6)). N: wild type (WT) = 96, GFP = 98, AP = 100, GA = 111, PR = 119, GR = 90. All flies in “b” and “c” were collected from at least 3 independent crosses