Fig. 3.

GCN5 is responsible for the succinylation of histone H3K79 on the HBV cccDNA minichromosome. a The interaction of GCN5 with cccDNA was measured by ChIP-qPCR from the livers from HBV-infected human liver-chimeric mice (n = 3). b HepaRG and HepG2-NTCP cells were infected with HBV at an MOI of 600 vp/cell. The deposition of GCN5 on cccDNA was measured by ChIP-qPCR in HBV-infected HepaRG and HepG2-NTCP cells. c, d HepaRG and HepG2-NTCP cells were infected with HBV at an MOI of 600 vp/cell and continuously transfected with siGCN5#1 (100 nM) at −4, 0, and 4 dpi. The succinylation of histone H3K79 on cccDNA minichromosome was verified by ChIP-qPCR 8 dpi in the cells. The mean ± SD of at least three experiments is shown. Statistically significant differences are indicated as follows: **P < 0.01; ns, no significance