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. 2020 Sep 7;18:143. doi: 10.1186/s12964-020-00646-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Wnt5b is involved in BC cell mesenchymal phenotype and stemness. a and b Expression of Vimentin, E-cadherin, Slug and Zeb1 was detected in MDA-MB-231 cells (a) and Hs578T cells (b) transfected with shCtrl or shWNT5B by Western blot. c and d Migration (c) and invasion (d) of Hs578T cells transfected with shCtrl or shWNT5B were analyzed by Wound healing or Transwell. e and f Heat map generated from CCLE database (e) and GSE12777 database (f) demonstrated the correlation of WNT5B with stemness-related genes in human BC cell lines. g Mammosphere formation in MDA-MB-231 cells transfected with shCtrl or shWNT5B was shown. h The fraction of Lgr5⁺ subpopulation in MDA-MB-231 cells transfected with shCtrl or shWNT5B was determined by flowcytometry. All experiments were carried out three times. Data are expressed as Mean ± s.e.m