Fig. 1.

Proteomics approach used to determine the LAP3 interactome. A: Workflow of the different experimental steps used in the analysis (see Materials and Methods for details). B: Hierarchical clustering of relative LFQ profiles of the putative LAP3 interactome identified (see Table 1 for annotation). Replicate GFP pulldown samples from Plasmodium berghei ookinetes expressing GFP-tagged LAP3 with or without in vitro crosslinking before pulldown are included, as well as two negative controls obtained from LAP3-KO parasites. LAP3 interactome proteins are largely absent in the non-crosslinked samples and negative controls.