Figure 2.

Genetic Background Influences Early Differentiation Capacity of Naive mESCs

(A) Schematic representation of undirected differentiation of mESC lines.

(B) Representative immunocytochemistry images for SOX1 after 6 days of undirected differentiation. Number in brackets indicates specific line shown.

(C) Quantification of flow cytometry data for SOX1 after 6 days of undirected differentiation. Data shown are from six independent differentiations for each genetic background, two lines per background. Error bars represent standard errors of the means. ^∗p < 0.05 and ^∗∗∗p < 0.0005 (two-tailed t test).

(D) Schematic representation of differentiation toward definitive endoderm.

(E) Representative immunocytochemistry images for SOX17 after 6 days of endoderm differentiation. Number in brackets indicates specific line shown.

(F) Quantification of flow cytometry data for SOX17 after 6 days of directed differentiation toward endoderm. Data shown are from seven independent differentiations for each genetic background, two lines per background. Error bars represent standard errors of the means. ^∗p < 0.05, ^∗∗p < 0.005, and ^∗∗∗p < 0.0005 (two-tailed t test).

(G) Representative bright-field images from each genetic background of cells differentiated for 12 days toward the primitive endoderm lineage. Number in brackets indicates specific line shown.

(H) Gene expression analysis of key markers after six days of directed differentiation toward primitive endoderm. Data shown are from five independent differentiations for each genetic background, two lines per genetic background. Error bars represent SD of the means. ^∗p < 0.05, ^∗∗p < 0.005, and ^∗∗∗p < 0.0005 (two-tailed t test).