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. 2020 Sep 1;32(9):108097. doi: 10.1016/j.celrep.2020.108097

Figure 1.

Figure 1

SWE Increases Whisker-Evoked Supra- and Sub-threshold Synaptic Responses in L2/3 Pyramidal Neurons

(A) Schematic of intrinsic optical signals and whole-cell recordings of L2/3 pyramidal neurons in full-whisker-experience (FWE) and single-whisker-experience (SWE) mice. ns, not significant.

(B) Statistical t-maps over 3 successive days. Scale bar, 500 μm.

(C) Averaged (± SEM) PW-evoked response area (normalized to the first session at day 0). Light/dark blue lines indicate individual FWE (n = 7)/SWE (n = 6) mice, respectively. Circle indicates mean. For SWE mice, whiskers are clipped after the first imaging session (dotted arrow).

(D) Left: single-cell examples of whisker-evoked responses (gray, single trials; dark and light blue, averaged traces from SWE and FWE mice, respectively). Square pulse lines, C2 whisker deflection (100 ms). Right: fraction of spiking neurons (top: SWE2 and SWE3 pooled together) and number of spikes per whisker deflection (bottom: spiking probability; median ± interquartile range).

(E) PW-evoked PSP grand average (all recorded cells averaged) ± SEM. Cells are recorded from FWE mice and SWE mice 48 h (SWE2) and 72 h (SWE3) after trimming. Square pulse lines, C2 whisker deflection (100 ms).

(F) Median (± interquartile range) resting membrane potential (Vrest), PSP peak amplitude, and integral. FWE, n = 20 cells; SWE2, n = 4 cells; SWE3, n = 9 cells.