Fig. 1.

Palmitoleic acid restores basal and glucose-stimulated insulin sensitivity in HFD-fed mice. (A) Body weight change during high fat diet (HFD)-feeding (n = 10–12); (B) adipose depot weights (n = 5–7); (C) fasting glucose, insulin levels and HOMA-IR (n = 5–7); (D) glucose levels during insulin tolerance test (ITT) and respective glucose clearance constant (KITT) (n = 5–7); (E) glucose changes during glucose tolerance test (GTT) and respective area under curve (AUC) (n = 5–7). Wild-type (WT) mice fed with a standard diet (SD) or high-fat diet treated with oleic acid (HFD OA) or palmitoleic acid (HFD POA). Data are presented as the mean ± SEM. *p < 0.05, ^⁎⁎p < 0.01, ^⁎⁎⁎p < 0.001 ****p < 0.0001 vs. WT SD; ^#p < 0.05 HFD POA vs. HFD OA. (One-way ANOVA followed by Bonferroni correction).