Fig. 1.

Alignment and analysis of the rapid alkalinisation factor (RALF) domain predicted in the F. graminearum sequence. A) Amino-acid sequence alignment of the predicted mature F. graminearum FgRALF (FGRAMPH1_01G16205), Arabidopsis thaliana AtRALF1 (AT1G02900) and F. oxysporum f.sp. lycopersici (FOXG_21151) proteins. Conserved residues are indicated in black. Cysteine residues predicted to form disulfide bonds are in red boxes. The conserved isoleucine residue essential for biological activity of AtRALF1 is in the blue box. B) Neighbour-Joining consensus tree of RALF proteins alignment from Arabidopsis thaliana and selected Fusarium species. The designated FgRALF, is shown in red text; RALF genes from other Fusarium species are shown in green text and the closely related RALF genes from Arabidopsis are shown in blue text.