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. 2020 Aug 31;11:536044. doi: 10.3389/fmicb.2020.536044

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Copyright © 2020 Song, Yang, Xue, Yang, Wu, Liu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The cellular localization of NcGrxs. (A) Strategy for construction of the NcGrx1-HA and NcGrx3-HA parasites. (B) The insertion of 3 × HA-tagged NcGrxs in parasites was confirmed by PCR. (C) Western blot confirmed the expression of HA-tagged NcGrxs in parasites. Actin was used as a control. (D) Immunofluorescence assays analysis of NcGrxs localization. αHA was used to detect the NcGrxs-HA (green), and SRS2 (red) served as a parasite surface marker (bar = 5 μm).