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. 2020 Aug 31;8:574394. doi: 10.3389/fcell.2020.574394

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Copyright © 2020 Jiang, Song, Jiang, Qiao, Yang, Wang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of ALDH1A1 and RARα as well as Ets1 were positive correlation in prostate cancer cells. Group-1 was Untreated group, Group-2 was Negative matched control transfection group, and Group-3, -4, -5 were Transfection group (Group 3–5 were transfected with siRNA-1, siRNA-2, siRNA-3). The expressions of ALDH1A1 (A), RARα (B), Ets1 (C) were detected by Western blotting, Statistical analysis on them (D–F). (G) Relative expression of ALDH1A1 mRNA was examined by RT-PCR. Data are expressed as the mean ± SD of three independent experiments. *P < 0.05 and **P < 0.01 compared with group-1.