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. 2020 Aug 31;8:574394. doi: 10.3389/fcell.2020.574394

FIGURE 3.

FIGURE 3

Screening and identification of ALDH1A1+ prostate cancer stem cell-like cells and their biological functions in vitro. Flow cytometry detected the expression of ALDH1A1 positive cells (A). The cells proliferative capacity was detected at 0, 24, 48, 72, 96 h by MTT assay (B). Plating efficiency was tested by plate clonality assay (C), and was colony forming efficiency was calculated (F). The expression of ALDH1A1 was detected by Immunocytochemistry (H, 200×, brown yellow granules indicate positive reaction). miR-ALDH1A1 was detected by Rt-PCR (D). The images of Transwell assay were recorded (I) which revealed the numbers of migration (E) and invasion (F) cells. To detected assess the presence and self-renewal ability of cells by sphere-formation Assay (G). The expression of ALDH1A1 were detected with immunohistochemistry (H). Plate clonality was used to detect the colony ability of DU145 cells (J). Data are expressed as the mean ± SD of three independent experiments. *P < 0.05 and **P < 0.01 compared with DU145.