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. 2020 Sep 10;39:183. doi: 10.1186/s13046-020-01659-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Loss of NEK2 enhances the radiosensitivity of cervical cancer cells. a Upper panel: HeLa and SiHa cells transfected with indicated siRNAs were exposed to radiation and harvested 4 h later. Immunostaining was performed to detect γ-H2AX foci formation. Lower panel: Quantification result of γ-H2AX foci was shown. Cell containing more than ten γ-H2AX foci was considered γ-H2AX-positive cell. ** P < 0.01, *** P < 0.001 (n = 3). Scale bar: 10 μm. b Upper panel: Representative pictures of neutral comet assay performed 4 h after IR exposure of NEK2-depleted or control cells. Lower panel: The olive tail moment was quantified and graphed. *** P < 0.001 (n = 3). Scale bar: 25 μm. c Left panel: HeLa and SiHa cells transfected with indicated siRNAs were exposed to radiation and harvested 4 h later. Immunostaining was performed to determine Rad51 foci formation. Right panel: Quantification result of Rad51 foci was shown. ** P < 0.01, *** P < 0.001 (n = 3). Scale bar: 10 μm. d Radiation sensitivity of HeLa and SiHa cells lacking NEK2. HeLa and SiHa cells transfected with indicated siRNAs were irradiated at indicated doses. The colonies were counted two weeks later. Data were shown as the mean ± SD. *** P < 0.001 (n = 3)