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. 2020 Sep 10;39:183. doi: 10.1186/s13046-020-01659-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — NEK2 silencing downreglates the expression of Wnt1 and attenuates the Wnt1/β-catenin signaling pathway. a Volcano plot of differentially expressed genes resulting from NEK2-depleted and control HeLa cells: the X-axis indicates the log2 transformed expression values, and the Y-axis represents the significance value after -log10 transformation. Red indicates upregulated differentially expressed genes, blue indicates downregulated differentially expressed genes, and gray indicates non-differentially expressed genes. b Heat map produced from RNA-seq data. HeLa cells were transfected with the indicated siRNAs for 48 h, and total mRNA was subjected to RNA-seq analysis. c List of several differentially expressed genes related to cell proliferation (Fold change > 2). d HeLa cells were transfected with the indicated siRNAs. After 48 h, the cells were harvested, and the levels of indicated mRNAs were measured by quantitative real-time PCR. ** P < 0.01, *** P < 0.001 (n = 3). e Knockdown of NEK2 led to decreased protein levels of Wnt1 and β-catenin in HeLa and SiHa cells (n = 3). f HeLa cells transfected with the indicated siRNAs for 48 h were collected, and the levels of indicated mRNAs were determined by quantitative real-time PCR. *** P < 0.001 (n = 3)