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. 2020 Aug 31;8:864. doi: 10.3389/fbioe.2020.00864

FIGURE 5.

FIGURE 5

Improved drill-hole bone healing in estrogen-deficient mice with IDG-SW3 cells. (A–D) After drill-hole surgery for 1 week, mice were sacrificed and the right femora were subjected to radiographic and histological examination (n = 5). (A) Representative X-ray images. (B) Grayscale analysis of the new bone formed in defect area. (C) Representative TRAP staining images of the defect area. Bar, 200 μm. (D) Quantification of the TRAP staining by osteoclast surface per bone surface (Oc.S/BS). (E–G) After drill-hole surgery for 2 weeks, mice were sacrificed and the right femora were subjected to radiographic and histological examination (n = 7). (E) 3D μ-CT images of the defect area. (F) μ-CT analysis of bone volume per tissue volume (BV/TV) in the intramedulla region. (G) Representative H&E staining images (left) and the relative polarized-light images (right) of the defect area. Data in (E) and (G) were derived from an identical femur. Bar, 500 μm. Data were represented as means ± s.e.m. Ordinary one-way ANOVA analysis. *P < 0.05. **P < 0.01. ***P < 0.001. SWL, standard white light; PWL, polarized white light.