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. 2020 Sep 10;20:443. doi: 10.1186/s12935-020-01529-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — miR-221-3p upregulation suppresses apoptosis in PC cells. Apoptosis was represented by a percentage (Q2 + Q3)/(Q1 + Q2 + Q3 + Q4). a After BxPC-3 cells were infected with control or mimic, apoptosis rates were tested with flow cytometry experiments. The apoptosis proportion was obviously decreased in BxPC-3 cells with miR-221-3p overexpression. b After BxPC-3 cells were infected with control or inhibitor, apoptosis rates were tested with flow cytometry experiments. The apoptosis proportion was increased in BxPC-3 cells with miR-221-3p overexpression. c After MIA PaCa-2 cells were infected with control or mimic, apoptosis rates were tested with flow cytometry experiments. The apoptosis proportion was decreased in MIA PaCa-2 cells with miR-221-3p overexpression. d After MIA PaCa-2 cells were infected with control or inhibitor, apoptosis rates were tested with flow cytometry experiments. The apoptosis proportion was increased in BxPC-3 cells with miR-221-3p overexpression. Three replicates were performed for this experiment. The results were analysed with t tests (unpaired), and significant p-values were defined as less than 0.05