Fig. 3.

Suppressor of cytokine signaling 1 (SOCS‐1) inhibition in dendritic cells (DCs) significantly restored their maturation suppressed by tumor‐conditioned medium (TCM). (a) After TCM pretreatment and maturation induction, the expression level of SOCS‐1 in DCs transfected with SOCS1 siRNA or NC siRNA was detected by Western blot. Relative density of SOCS1 was determined by densitometry of the blots; ^*** P < 0·001 versus the negative control (NC) siRNA group. (b) The influence of SOCS1 inhibition on the expression frequency of CD80, CD86 and CD83 was detected using flow cytometry. (c) Flow cytometry profiles were shown as the percentage of positive cells; ^*** P < 0·001 versus the NC siRNA group. (d) Secretion of interleukin (IL)‐10, IL‐12p70 and transforming growth factor (TGF)‐β in the supernatant was detected by enzyme‐linked immunosorbent assay (ELISA); ^*** P < 0·001 versus the NC siRNA group.