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. 2020 Jun 30;202(1):47–59. doi: 10.1111/cei.13476

Fig. 4.

Fig. 4

Suppressor of cytokine signaling 1 (SOCS‐1) inhibition in dendritic cells (DCs) significantly restored T cell viability and T helper type 1 (Th1) response suppressed by tumor‐conditioned medium (TCM). (a) Pretreated DCs and T cells were co‐cultured at different ratios for 7 days, and the T cell viability was detected by 3‐(4,5‐dimethyl‐thiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay; n = 3, * P < 0·05, ** P < 0·01 versus the DC + T cell + TCM + negative control (NC) group. (b) T cell viability at a DC : T ratio of 1 : 1 was measured by MTT; n = 3, ** P < 0·01 versus the DC + T cell + TCM + NC group. (c) Secretion level of interferon (IFN)‐γ at a DC : T ratio of 1 : 1 after co‐culture for 7 days was measured by enzyme‐linked immunosorbent assay (ELISA); n = 3, ** P < 0·01 versus the DC + T cell + TCM + NC group.