Fig. 5.

Tumor‐conditioned medium (TCM) pretreatment inhibited nuclear factor kappa B (NF‐κB) signaling pathway of dendritic cells (DCs) and suppressor of cytokine signaling 1 (SOCS‐1) inhibition significantly rescued it. (a) DCs transfected with or without SOCS1 siRNA were cultured in the presence or absence of tumor‐conditioned medium (TCM). The expression of p65 in cytosol and nucleus were determined using Western blot. Lamin B was used as the internal references of nucleus, and β‐actin was used as the internal references of cytosol. (b) Relative densities of SOCS1 and p65 were determined by densitometry of the blots; ^*** P < 0·001 versus the negative control (NC) siRNA group, ^### P < 0·001 versus the mDC group. (c) The luciferase reporters driven by NF‐κB response elements were transfected into DCs for 24 h, and then cells were transfected with or without SOCS1 siRNA and cultured in the presence or absence of TCM. Luciferase activity was analyzed by the dual‐luciferase reporter assay system; ^** P < 0·001 versus the NC siRNA group; ^### P < 0·001 versus the mDC group.