Table 2.
List of antibodies used in immunoblots and flow cytometry
| Antibody | Species | Source | Cat. no. | Dilution |
|---|---|---|---|---|
| Primary antibodies | ||||
| Anti‐β‐actin | Rabbit, monoclonal | Sigma‐Aldrich, St Louis, MO, USA | SAB5500001 | 1 : 1000 |
| Anti‐SOCS1 | Rabbit, polyclonal | Sigma‐Aldrich, St Louis, MO, USA | AV42147 | 1 : 1000 |
| Anti‐CRIP1 | Rabbit, polyclonal | Sigma‐Aldrich, St Louis, MO, USA | SAB2105783 | 1 : 1000 |
| Anti‐RARRES3 | Mouse, polyclonal | Sigma‐Aldrich, St Louis, MO, USA | SAB1406374 | 1 : 1000 |
| Anti‐NF‐κB p65 | Rabbit, polyclonal | Sigma‐Aldrich, St Louis, MO, USA | SAB4502610 | 1 : 1000 |
| Anti‐lamin B | Rabbit, polyclonal | Sigma‐Aldrich, St Louis, MO, USA | SAB1306342 | 1 : 1000 |
| Anti‐CD80‐FITC | Mouse, monoclonal | Abcam, Cambridge, MA, USA | ab18279 | 1 : 100 |
| Anti‐CD83‐FITC | Mouse, monoclonal | Abcam, Cambridge, MA, USA | ab234233 | 1 : 100 |
| Anti‐CD86‐PE | Mouse, monoclonal | Abcam, Cambridge, MA, USA | ab234226 | 1 : 100 |
| Secondary antibodies | ||||
| Anti‐rabbit (HRP) | Goat | Sigma‐Aldrich, St Louis, MO, USA | A0545 | 1 : 80000 |
| Anti‐mouse (HRP) | Rabbit | Sigma‐Aldrich, St Louis, MO, USA | A9044 | 1 : 80000 |
SOCS1 = suppressor of cytokine signaling 1; CRIP1 = cysteine rich protein 1; NF‐kB = nuclear factor kappa B; RARRES3 = retinoic acid receptor responder (tazarotene‐induced) 3; FITC = fluorescein isothiocyanate; PE = phycoerythrin; HRP = horseradish peroxidase.