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. 2020 Sep 9;26:86. doi: 10.1186/s10020-020-00201-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — THRIL inhibits proliferation and promoted apoptosis through binding with FUS (a left) The expression of THRIL in CAD EPCs cell nucleus and cytoplasm was measured through northern blotting. (a right) The expression of FUS in CAD EPCs cell nucleus and cytoplasm was measured through western blotting. (b-d) The direct interactions between THRIL and FUS was confirmed through RNA electrophoretic mobility shift assays (RNA EMSA) and RNA immunoprecipitation (RIP) assays. (e) The effects of THRIL over-expression on cell viability of CAD EPCs was reversed through knockdown of FUS. (f) The effects of over-expression of THRIL on cell apoptosis of CAD EPCs was reversed through knockdown of FUS. NC: EPCs isolated from CAD patients; All the transfection experiments were performed in EPCs isolated from CAD patients. Data are presented as mean ± SD. *P < 0.05. The data shown represent three separate experiments performed in triplicate in each experiment (mean ± the standard deviation [SD])