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. 2020 Aug 31;10:455. doi: 10.3389/fcimb.2020.00455

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At least a portion of this work is authored by Eric Calvo, Hugo O. Valdivia, Karin Escobedo-Vargas, Luz Romero, Maria Bosantes, Michael L. Fisher, Gissella M. Vásquez and Audrey E. Lenhart on behalf of the U.S. Government and, as regards Drs. Calvo, Valdivia, Escobedo-Varga, Romero, Bosantes, Fisher, Vásquez and Lenhart and the U.S. Government, is not subject to copyright protection in the United States. Foreign and other copyrights may apply.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunoblot of An. darlingi SGA. Pooled serum from uninfected individuals exposed to An. darlingi bites was used to identify immunogenic proteins. (A) Silver stain of whole SGA. (B) Immunoblot.