Figure 7.

Peli1 does not regulate the response of resident immune cells to TLR3 activation in the murine lung. Explanted lung tissue from age- and sex-matched Peli1^+/+ (n = 3) and Peli1^−/− mice (n = 3) was stored at 4°C in isotonic saline and transported to Newcastle University for processing. Precision cut lung sections were cultured in vitro and rested for 48 h prior to treatment with poly(I:C) or LPS for 24 h at the concentrations indicated. Cell culture supernatants were analyzed for IL-6 (A), CXCL10 (B), KC (C), CCL2 (D), CCL5 (E), and TNFα (F) release using MSD electrochemiluminescence detection. Data shown are mean ± SEM. No statistically significant differences were found.