Fig. 2.

Integration of plastid rps7 into the intron of nuclear-encoded thioredoxin gene in Passiflora. (A) Schematic diagram (not to scale) depicts the insertion of plastid rps7 into the intron of thioredoxin (trx-m3) that contains TP known for plastid localization. Gray boxes indicate the exons of the trx-m3 gene and the black line in between indicates the intron. The first exon of trx-m3 gene contains TP. White box represents the plastid rps7. Alternative splicing is shown in dotted arrows. Blue and red arrows represent the gene product of alternative splicing and localization of the product to the plastid, respectively. Arrows (a, b, and c) below the chimeric rps7-trx-m3 indicate the location annealing sites of primers designed to amplify the gene product. The figure is not drawn to scale. (B) PCR amplifications of the chimeric rps7-trx-m3 in Passiflora pittieri with the primers designed in figure (A). Lane 1, 1 kb DNA ladder (N3232L New England Biolabs, Inc); Lane 2, PCR product with primer set a and b; and Lane 3, PCR product with primer set a and c as indicated in (A). (C) Passiflora pittieri chimeric rps7-trx-m3 as a representation for all other Passiflora species. The three exons of the gene are annotated in yellow. Intron 5′ and 3′ splice sites are boxed in gray. Abbreviations, Nu, nucleus; Pt, plastid, Mt, mitochondrion.