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. 2020 Jun 15;12(8):1313–1329. doi: 10.1093/gbe/evaa123

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© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 4. — Nuclear-encoded RPL20 isoforms in Passiflora. The NCBI CD database was used for CD prediction. (A) Putative mitochondrial RPL20 (RPL20-1) in Passiflora containing RNA-binding site as well as binding sites for other ribosomal subunits. (B) Putative plastid RPL20 (RPL20-2) in Passiflora with predicted binding sites for ribosomal subunits. (C) Mapping of P. oerstedii rpl20-2 transcript against the P. edulis BAC clone Pe84M23 indicates the presence of an intron. (D and E) PCR amplifications to verify intron presence in rpl20-1 and rpl20-2 genes. Lane 1, 1 kb DNA ladder (N3232L New England Biolabs, Inc); Lane 2, Passiflora pittieri; Lane 3, P. contracta; Lane 4, P. oerstedii; Lane 5, P. tenuiloba; Lane 6, P. auriculata; and Lane 7, P. biflora.