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. 2020 Jun 15;10(8):1521–1533. doi: 10.1016/j.apsb.2020.06.005

Figure 3.

Fig. 3

Transfection (A and B) and anti-inflammatory activity (C–E) in vitro. BCL-6 expression in RAW 264.7 cells determined by (A) WB assay and (B) quantitative analysis (n = 3, ∗∗P < 0.01). (C) ROS, (D) NF-κB, and (E) TNF-α expression in RAW 264.7 cells (mean ± SD, n = 3, ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001). The internal control for normalizing protein expression was β-actin. Formulations: 1, saline; 2, Naked RNA; 3, BNRs; 4, BNRplex; 5, SA-BNRplex. The cells were incubated with formulations at concentrations of 60 μg/mL for baicalein, 10 μg/mL for atorvastatin, or 100 nmol/L for anti-miR 155 for 24 h at 37 °C.