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. 2019 Dec 16;10(8):1476–1491. doi: 10.1016/j.apsb.2019.12.008

Figure 2.

Figure 2

Target engagement of USP28 inhibitors in cellular environment. (A) Treatment of HGC-27 and MGC-803 cells with 0.75 μmol/L of compound 19 for 3 h. Cells were harvested and subsequently re-suspended in PBS. The cell suspension was heated for 3 min at the corresponding temperature, and the USP28 content in each sample was then determined by Western blotting. (B) and (C) Densitometry analysis of USP28 content. Data are the mean ± standard deviation (SD). All experiments were performed for three times.