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. 2019 Dec 16;10(8):1476–1491. doi: 10.1016/j.apsb.2019.12.008

Figure 3.

Figure 3

Effects of compound 19 on the cell viability of gastric cancer cells and GES-1. (A) USP28 expression in four gastric cell lines. (B) The cytotoxic effect of compound 19 on HGC-27, MGC-803, MKN45 and GES-1. (C) USP28 expression was determined by Western blotting after 48 h treatment using USP28 siRNA (50 nmol/L) transfection in HGC-27 cells, and GAPDH was used as the loading control. (D) Cell viability upon USP28 siRNA treatment. (E) The effect of compound 19 on the cell viability in USP28 knockdown HGC-27 cells with different concentrations (0, 1.56, 3.12, 6.25, 12.5, 25, 50 and 100 μmol/L). ∗P < 0.05 and ∗∗P < 0.01 were considered statistically significant. All experiments were performed for three times.