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Expression levels of USP28, LSD1 and total c-Myc protein in HGC-27 cells following treatment with compounds 12 or 19 for 3 h. (A) Chemical structures of compounds 12 and 19. (B) Inhibition curves of compounds AZ1, 12 and 19 to USP28 in vitro. (C) and (D) The expression levels of USP28, LSD1 and total c-Myc were then determined by Western blotting using GAPDH as loading control.
