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. 2019 Dec 16;10(8):1476–1491. doi: 10.1016/j.apsb.2019.12.008

Figure 5.

Figure 5

USP28 inhibitor induced cellular degradation of USP28, LSD1 and total c-Myc. (A)–(C) Cells were treated with CHX, MG-132, following treatment with 19, 12 and DMSO, respectively. HGC-27 cells were pretreated with CHX (100 μg/mL) and MG132 (20 μmol/L), and then exposed to 19 (20 μmol/L), 12 (20 μmol/L) or DMSO. Cells were lysed at the indicated time points (from 0 to 360 min). The expression levels of USP28, LSD1 and total c-Myc were determined by Western blotting using GAPDH as the loading control.