FIGURE 2.

Phylogenetic comparison of the phyllogen family. (a) and (b) Neighbour‐joining phylogenetic tree based on 16S rRNA between primers SN910601/SN910502 (a) and full‐length phyllogen (b) gene nucleotide sequences. Sequences were aligned with the MUSCLE multiple alignment algorithm and analysed with a complete‐deletion option. Acholeplasma laidlawii strain PG‐8A was used to root the tree of 16S rRNA. Numbers at the nodes represent the percentage of bootstrap values obtained for 1,000 replicates (only values >70% are shown). Bars indicate the number of nucleotide substitutions per site. For the C‐terminus truncated phyllogens due to the premature stop codon (indicated by ^Ψ), the nucleotide regions after the premature stop codons were also included in the MUSCLE alignment. Full strain names and GenBank accession numbers are listed in Table S1. Background colours define related “Candidatus Phytoplasma” species