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Effect of sex on D-loop methylation levels in total sample (n = 114, a), in control subjects (n = 51, b), and in ALS patients (n = 63, c). Effect of sex on mtDNA copy number in total sample (n = 114, d), in control subjects (n = 51, e), and in ALS patients (n = 63, f). Statistical analysis was performed by means of ANCOVA, using age at sampling as covariate. Data are expressed as means ± standard deviation (SD). *p < 0.05; **p < 0.01
