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. 2019 Dec 27;10(8):1492–1510. doi: 10.1016/j.apsb.2019.12.013

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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(A) List of enriched KEGG terms of differentially expressed genes in U251 cells after incubation with ent-4g. (B) Analysis of enriched KEGG terms based on statistical significance (logarithmic Q-value) and enrichment factors. (C) Apoptosis in U251 cells treated with ent-4g based on Annexin V/PI dual staining. (D) Cell cycle analysis of U251 cells treated with ent-4g. All values are the average from three independent experiments. (E) Western blotting and analysis of CDK4-cyclin D1 and downstream proteins in U251 cells after 24-h incubation with ent-4g. (F) Western blotting and analysis of MDM2, P53 and apoptosis-related proteins in U251 cells after 24-h incubation with ent-4g. All values are the average of three experiments. NS, normal saline.