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. 2019 Dec 27;10(8):1492–1510. doi: 10.1016/j.apsb.2019.12.013

Figure 5.

Figure 5

In vivo anti-cancer effect of ent-4g on U251 xenografts in mice. (A) Tumor volume after administration of ent-4g (30 mg/kg), nutlin-3a (30 mg/kg) or palbociclib (30 mg/kg). (B) Tumor weight at the end of therapy. (C) Body weight at different times after treatment. (D) Representative images of immunohistochemical analysis of MDM2, P53, P21, pCDK4, pRB, and Ki-67, as well as of immunofluorescence analysis of TUNEL staining for apoptosis. (E) Percentages of cells staining positively for target proteins or TUNEL stain. NS, normal saline; N + P, nutlin-3a + palbociclib. Data were expressed as mean ± SD, ∗P < 0.05, ∗∗P < 0.01.