Fig. 4.

EIF4G1 knockdown facilitated the radiosensitivity in PCa cells. a, b The relative mRNA and protein expression of EIF4G1 were determined in 22RV1 and DU145 cells transfected with si-EIF4G1#1, si-EIF4G1#2, si-EIF4G1#3 or si-NC by qRT-PCR and western blot, respectively. c–i Cells were transfected with si-NC or si-EIF4G1#3. c, d MTT assay was used to examine cell viability of transfected 22RV1 (c) and DU145 (d) cells after 6 Gy irradiation. e, f The colony survival fractions were measured in transfected 22RV1 (e) and DU145 (f) cells subjected to irradiation. g Cell apoptosis was determined in transfected 22RV1 and DU145 cells after 6 Gy radiotherapy. h, i The protein expression of Cyclin D1, Bcl-2, and Bax was examined in transfected 22RV1 (h) and DU145 (i) cells after 6 Gy irradiation. ^*P < 0.05