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. 2020 Sep 11;11:393. doi: 10.1186/s13287-020-01914-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Small molecule-based protocol for the differentiation of hepatocytes from induced pluripotent stem cells. a Schematic diagram showing the three-phase differentiation process. b Representative images showing sequential morphological changes at each stage of the differentiation. iPSC, induced pluripotent stem cell; ME, mesendoderm; DE, definitive endoderm; HP, hepatic progenitor; HLC, hepatocyte-like cell. Magnification: × 10, Insert: × 40, Scale bar: 400 μm. c Representative immunocytochemistry images demonstrating the expression of key markers at each stage of the differentiation. The percentage of marker positive cells, presented as mean ± SD of three independent experiments, is listed at the bottom under each marker. Scale bar: 400 μm