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. 2020 Sep 11;11:393. doi: 10.1186/s13287-020-01914-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Principal component analysis (PCA) plot showing the differentiation track (dotted line) and similarities/dissimilarities among the samples. The two axes PC1 and PC2 represent the first two principal components identified by the analysis. The percentage contribution of each component to the overall source of variation is included in the parentheses following the component name. Color codes of different cell types are shown at the top-right corner. The timing of the small molecule-driven and growth factor-driven differentiation is denoted by D (day) followed by the number of the day. iPSC, induced pluripotent stem cell; DE, definitive endoderm; HP, hepatic progenitor; HLC, hepatocyte-like cell; PHHs, primary human hepatocytes. HLC_Wruck and HLC_Yang are reference datasets