Fig. 3.

MiR-214-3p inhibitor inhibits porcine GC proliferation. MiR-214-3p antagomir or NC transfected into cells harvested after 24 h. a Knock-down efficiency of miR-214-3p after transfection with miR-214-3p antagomir compared to NC; b Flow cytometry determines cell percentages in different cycle phases; c Cell cycle analysis statistical results; d EdU staining assays proliferous cell quantities. Positive cells stained by EdU (red) and total cell nucleus stained with Hoechst (blue); e Results represented as red/blue cell nuclei percentages; f CCK-8 assay detects cell viability after 24-h transfection as absorbance value at 450 nm after incubation with 10% CCK-8 solution for 4 h; g RT-qPCR detects cell cycle genes, Cyclin B, Cyclin E, Cyclin D, CDK4 after 24-h transfection; h Western blot analysis of cell cycle genes; (I) Quantification of Western blot analysis of Cyclin B, Cyclin D, Cyclin E, CDK4. Note: Data represent mean ± SEM of three independent experiments; * P < 0.05, ** P < 0.01