Fig. 7.

MiR-214-3p targets NR5A1 during GC estradiol synthesis. a miR-214-3p binding site within NR5A1 3′-UTR predicted by RNAhybrid; b target site of miR-214-3p within porcine NR5A1 mRNA 3’UTR and mutational site of NR5A1 3’UTR; c Dual luciferase assay via co-transfection of miR-214-3p agomir and wild-type vectors or mutant vectors. Relative luciferase activity represented by RLUC/FLUC; d Relative NR5A1 mRNA expression levels after treatment with miR-214-3p agomir; e Relative NR5A1 mRNA expression levels after treatment with miR-214-3p antagomir; f Western blot analysis of NR5A1 protein expression after treatment with miR-214-3p agomir; h Western blot analysis of NR5A1 protein expression after treatment with miR-214-3p antagomir; g, i Quantification of NR5A1 protein levels. Note: Data are mean ± SEM of three independent experiments; * P < 0.05, ** P < 0.01