Fig. 4.

Simulation of the effect of sequencing depth on dissimilarity between replicates. a Relative abundance distribution for the microbial community being sampled. b ASVs detected in samples having different sequencing depths. Dark red color indicates that the ASV was detected. Three samples are shown for each sequencing depth. c Average pairwise dissimilarities between replicate samples at each sequencing depth. The shaded regions show the standard deviations (n = 15). Jaccard- and Bray-Curtis dissimilarities are shown as squares and circles, respectively