Fig. 2.

Mouse monoclonal antibodies bind spinal cord derived hSOD1 aggregates. a Immunohistochemical staining of proteinase-resistant hSOD1 aggregates in the ventral horn of spinal cord sections from C57BL/6J non-Tg control (Wt); 200-day-old pre-symptomatic hSOD1^G85R Tg; and 400-day terminal stage Tg hSOD1^G85R mice, using mAbs raised against the aa 57–72 peptide (hSOD1^57–72) or aa 131–153 (hSOD1^131–153). Scale bar = 40 μm. b Dot blot of filter-captured aggregates from spinal cords of end-stage disease hSOD1^G85R and hSOD1^G93A Tg mice, probed with the indicated αSOD1 mAbs. Polyclonal rabbit antibodies raised against the same peptides (rbAb hSOD1^57–72; rbAb hSOD1^131–153) were included as positive controls. c Graph represents relative staining intensities of the hSOD1^G85R aggregates. To assess aggregate/mAb complex stability, filters were either washed for 1 h at 23 °C or exposed to extended 24 h washing at 23 °C or 37 °C (n = 3 for each condition). Data presented as mean ± SD