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. 2020 Sep 14;16:334. doi: 10.1186/s12917-020-02549-3

Fig. 3.

Fig. 3

Neutralization assay using mAbs for PCV2 genotypes. 200 TCID50 of QIA215 (○), QIA418 (□), QIA169 (●), and QIA244 (■) were neutralized withdiluents (1/200 to 1/3200) of mAb-1 (a), mAb-2 (b), mAb-3 (c), mAb-4 (d), mAb-5 (e), and mAb-6 (f), and the neutralized mixtures were infected on PK15 cells. The fluorescent intensity of PCV2-positve cells at day 5 of post-infection was determined as a %VN by immunostaining with mAb-2 having the reactivity against all PCV2 genotypes. *Significantly different from control at p < 0.05