Table 2.
Intra-day and inter-day precision and accuracy for UPLC-MS/MS assay of standards in fermented DBT
| Analyte | Spiked (μg/mL) | Intra-day (n = 6)a | Inter-day (n = 18)b | |||||
|---|---|---|---|---|---|---|---|---|
| Measured (μg/mL) | Precision (RSD%) | Accuracy (RE%) | Measured (μg/mL) | Precision (RSD%) | Accuracy (RE%) | |||
| Cal-O-β-glu | 0.07 | 0.07 | 4.52 | 2.77 | 0.07 | 4.09 | 4.24 | |
| 1.84 | 1.91 | 0.84 | 4.16 | 1.98 | 2.30 | 7.93 | ||
| 16.60 | 16.30 | 2.04 | − 1.97 | 16.70 | 1.74 | − 0.14 | ||
| Ononin | 0.07 | 0.07 | 2.83 | − 2.30 | 0.06 | 6.09 | − 5.03 | |
| 1.84 | 2.03 | 1.92 | 10.20 | 2.09 | 2.48 | 13.90 | ||
| 16.60 | 14.80 | 2.32 | − 12.80 | 14.90 | 1.44 | − 10.30 | ||
| Calycosin | 0.07 | 0.07 | 2.06 | 4.42 | 0.06 | 8.51 | − 10.80 | |
| 1.84 | 2.05 | 1.32 | 11.50 | 2.04 | 2.56 | 11.20 | ||
| 16.60 | 15.50 | 2.21 | − 7.58 | 16.00 | 1.10 | − 6.51 | ||
| Formononetin | 0.07 | 0.07 | 2.61 | 3.93 | 0.06 | 2.44 | − 6.58 | |
| 1.84 | 1.99 | 1.91 | 7.99 | 1.99 | 2.63 | 7.88 | ||
| 16.60 | 15.60 | 2.20 | − 6.87 | 15.80 | 1.02 | − 4.90 | ||
aThe intra-day analysis refers to the sample examined for 6 replicates within one day
bThe inter-day analysis refers to the sample examined in duplicates over 3 consecutive days
Cal-O-β-glu calycosin-7-O-β-D-glucoside