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. 2020 Sep 10;19:1533033820947489. doi: 10.1177/1533033820947489

Figure 2.

Figure 2.

DFMO and Thymoquinone synergize to induce apoptosis in Jurkat cells. To evaluate the synergistic effect on apoptosis, cells were treated with either DFMO at 1 mM for 48 h or TQ at 10 μM or incubated with 1 mM of DFMO for 24 h before adding TQ at (10 μM) for additional 24 h (A & B). To confirm the synergistic effect of TQ and DFMO, cells were treated with either DFMO at 0.5 mM for 48 h or TQ at 20 μM or incubated with 0.5 mM of DFMO for 24 h before adding TQ at (20 μM) for additional 24 h. Apoptosis in Jurkat cells was assessed by flow cytometry using the Annexin V/7AAD staining apoptosis assay (A, B, C & D). Values are shown as means ± S.E.M. (n = 3); *, p < 0.05, ***, p < 0.001, ****, p < 0.0001, ###, p < 0.001, ####, p < 0.0001, &&, p < 0.01, &&&&, p < 0.0001 versus respective control.